产品详情
产品名称XRN2 Antibody
来源种属Mouse
克隆性Monoclonal
纯化ProA affinity purified
应用WB, IHC
种属反应性Hu
免疫原描述Recombinant protein
别名5' 3' exoribonuclease 2 antibody
DHM1 like protein antibody
DHP protein antibody
XRN 2 antibody
数据库入口号Swiss-Prot#:Q9H0D6
计算分子量109 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:200-1:500
Western blot analysis of XRN2 on human XRN2 recombinant protein using anti-XRN2 antibody at 1/1,000 dilution.
Western blot analysis of XRN2 on HEK293 (1) and XRN2-hIgGFc transfected HEK293 (2) cell lysate using anti-XRN2 antibody at 1/1,000 dilution.
Western blot analysis of XRN2 on different cell lysate using anti-XRN2 antibody at 1/1,000 dilution. Positive control�� Lane1: ?Raw264.7 Lane2: HEK293 Lane3: NTERA-2 Lane4: LNcap Lane5: HepG2 Lane6: HEK293 Lane7: Hela?
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-XRN2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti- XRN2 antibody. Counter stained with hematoxylin.
背景
Degradation of mRNA is a critical aspect of gene expression that occurs via the exoribonuclease. Exoribonuclease 2 (XRN2) is the human homologue of the Saccharomyces cerevisiae RAT1, which functions as a nuclear 5' to 3' exoribonuclease and is essential for mRNA turnover and cell viability. XRN2 also processes rRNAs and small nucleolar RNAs (snoRNAs) in the nucleus. XRN2 moves along with RNA polymerase II and gains access to the nascent RNA transcript after the endonucleolytic cleavage at the poly(A) site or at a second cotranscriptional cleavage site (CoTC). CoTC is an autocatalytic RNA structure that undergoes rapid self-cleavage and acts as a precursor to termination by presenting a free RNA 5' end to be recognized by XRN2. XRN2 then travels in a 5'-3' direction like a guided torpedo and facilitates the dissociation of the RNA polymerase elongation complex.
背景文献
1. Haas G ?et al.?Identification of factors involved in target RNA-directed microRNA degradation.?Nucleic Acids Res?44:2873-87 (2016). 2. Gaulke CA ?et al.?Intestinal Epithelial Barrier Disruption through Altered Mucosal MicroRNA Expression in Human Immunodeficiency Virus and Simian Immunodeficiency Virus Infections.?J Virol?88:6268-80 (2014).