产品详情
产品名称Cyclin D1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性SA38-08
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名AI327039 antibody
B cell CLL/lymphoma 1 antibody
B cell leukemia 1 antibody
B cell lymphoma 1 protein antibody
B-cell lymphoma 1 protein antibody
BCL 1 antibody
BCL-1 antibody
BCL-1 oncogene antibody
BCL1 antibody
BCL1 oncogene antibody
ccnd1 antibody
CCND1/FSTL3 fusion gene, included antibody
CCND1/IGHG1 fusion gene, included antibody
CCND1/IGLC1 fusion gene, included antibody
CCND1/PTH fusion gene, included antibody
CCND1_HUMAN antibody
cD1 antibody
Cyl 1 antibody
D11S287E antibody
G1/S specific cyclin D1 antibody
G1/S-specific cyclin-D1 antibody
Parathyroid adenomatosis 1 antibody
PRAD1 antibody
PRAD1 oncogene antibody
U21B31 antibody
数据库入口号Swiss-Prot#:P24385
计算分子量34 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of Cyclin D1 on different lysates using anti-Cyclin D1 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: SH-SY-5Y
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
ICC staining Cyclin D1 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cyclin D1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
背景文献
1. Totta, P. et al. 2015. Clathrin heavy chain interacts with estrogen receptor α and modulates 17β-estradiol signaling. Molecular endocrinology (Baltimore, Md.). : me20141385.
2. Luo, Y. et al. 2015. Lycorine induces programmed necrosis in the multiple myeloma cell line ARH-77. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 36: 2937-45.
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et al,Long non-coding RNA TRIM52-AS1 sponges microRNA-577 to facilitate diffuse large B cell lymphoma progression via increasing TRIM52 expression. In Hum Cell
on 2022 Jul by Fang Zhao, Shucheng Li,et al..PMID:35676608,
, (2022),
PMID: 35676608
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