产品详情
产品名称Integrin beta-1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性SR30-03
纯化ProA affinity purified
应用WB, IHC, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名beta1 integrin antibody
CD29 antibody
Fibronectin receptor subunit beta antibody
FNRB antibody
Glycoprotein IIa antibody
GP IIa antibody
GPIIA antibody
Integrin beta-1 antibody
integrin VLA-4 beta subunit antibody
Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody
ITB1_HUMAN antibody
ITGB1 antibody
MDF2 antibody
MSK12 antibody
OTTHUMP00000019420 antibody
Very late activation protein, beta polypeptide antibody
VLA BETA antibody
VLA-4 subunit beta antibody
VLA-BETA antibody
VLAB antibody
VLAbeta antibody
数据库入口号Swiss-Prot#:P05556
计算分子量150 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC:1:50-1:200
FC: 1:50-1:100
Western blot analysis of Integrin beta-1 on human liver lysates using anti-Integrin beta-1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Integrin beta-1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Integrin beta-1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Integrin beta-1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Integrin beta-1 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Hela cells with Integrin beta-1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
Integrins are heterodimers composed of noncovalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, collagen and vitronectin. Certain integrins can also bind to soluble ligands, such as fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.
背景文献
1. Szeto HH et al. Improving mitochondrial bioenergetics under ischemic conditions increases warm ischemia tolerance in the kidney. Am J Physiol Renal Physiol 308:F11-21 (2015).
2. Bayer ML et al. Release of tensile strain on engineered human tendon tissue disturbs cell adhesions, changes matrix architecture, and induces an inflammatory phenotype. PLoS One 9:e86078 (2014).