产品详情
产品名称Caspase-3 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性SR03-01
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Rt
免疫原描述recombinant protein
别名A830040C14Rik antibody Apopain antibody CASP-3 antibody CASP3 antibody CASP3_HUMAN antibody Casp3a antibody Caspase 3 antibody Caspase 3, apoptosis-related cysteine peptidase antibody Caspase 3, apoptosis-related cysteine protease antibody Caspase 3, apoptosis-related cysteine protease a antibody Caspase-3 subunit p12 antibody CC3 antibody CPP-32 antibody CPP32 antibody CPP32B antibody Cysteine protease CPP32 antibody EC 3.4.22.56 antibody LICE antibody mldy antibody OTTHUMP00000165052 antibody OTTHUMP00000165053 antibody OTTHUMP00000165054 antibody PARP cleavage protease antibody Procaspase3 antibody Protein Yama antibody SCA 1 antibody SCA-1 antibody SREBP cleavage activity 1 antibody Yama antibody
数据库入口号Swiss-Prot#:P42574
计算分子量32 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:100
FC:1:20-1:50
Western blot analysis of Caspase-3 on different cell lysates using anti-Caspase-3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: 293
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
ICC staining Caspase-3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.
背景文献
1. Li H et al. Protective effect of ginsenoside Rg1 on lidocaine-induced apoptosis. Mol Med Rep 9:395-400 (2014). 2. Cejkova J et al. Suppression of alkali-induced oxidative injury in the cornea by mesenchymal stem cells growing on nanofiber scaffolds and transferred onto the damaged corneal surface. Exp Eye Res 116:312-23 (2013).
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et al,Potential role of the cAMP/PKA/CREB signallIng pathway In hypoxic preconditionIng and effect on propofol?Induced neurotoxicity In the hippocampus of neonatal rats.In Mol Med Rep on 2019 Aug by Guan R, Lv J, et al..PMID:31257533,
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PMID: 31257533