产品详情
产品名称ICAM1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性ST0487
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Hu
免疫原描述recombinant protein
别名Antigen identified by monoclonal antibody BB2 antibody BB 2 antibody BB2 antibody CD 54 antibody CD_antigen=CD54 antibody CD54 antibody Cell surface glycoprotein P3.58 antibody Human rhinovirus receptor antibody ICAM 1 antibody ICAM-1 antibody ICAM1 antibody ICAM1_HUMAN antibody intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody Intercellular adhesion molecule 1 antibody Major group rhinovirus receptor antibody MALA 2 antibody MALA2 antibody MyD 10 antibody MyD10 antibody P3.58 antibody Surface antigen of activated B cells, BB2 antibody
数据库入口号Swiss-Prot#:P05362
计算分子量89 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of ICAM1 on different lysates using anti-ICAM1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HUVEC Lane 3: Raji
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.
ICC staining ICAM1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ICAM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Cell adhesion molecules (CAMs) are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play important, yet separate, roles in embryogenesis and development. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the beta2alpha1 and beta2alphaM Integrins. ICAM-2 functions as a ligand for lymphocyte function-associated antigen-1 (LFA-1) and is involved in leukocyte adhesion. ICAM-3 is highly expressed on the surface of human eosinophils and, when bound to ligand, may inhibit eosinophil inflammatory responses and survival. ICAM-4, also known as LW glycoprotein, interacts with Integrins alphaLbeta2, alphaMbeta2, alpha4beta1, the alphaV family and alphaIIbbeta3, and selective binding to different integrins may be relevant to the pathology in a number of red blood cell associated diseases. Lastly, ICAM-5, expressed on telencephalic neurons, binds CD11a/CD18 and thus may act as an adhesion molecule for leukocyte binding in the central nervous system.
背景文献
1. Grover JR et al. Basic motifs target PSGL-1, CD43, and CD44 to plasma membrane sites where HIV-1 assembles. J Virol 89:454-67 (2015). 2. Rouault C et al. Roles of chemokine ligand-2 (CXCL2) and neutrophils in influencing endothelial cell function and inflammation of human adipose tissue. Endocrinology 154:1069-79 (2013).