产品详情
产品名称AKT1/2/3 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性ST48-09
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名AKT antibody
AKT1 antibody
AKT1 kinase antibody
AKT1m antibody
AKT2 antibody
AKT2 kinase antibody
Akt3 antibody
AKT3_HUMAN antibody
CAKT antibody
CWS6 antibody
DKFZp434N0250 antibody
HIHGHH antibody
kinase Akt1 antibody
MGC99656 antibody
MPPH antibody
Murine thymoma viral (v-akt) homolog 2 antibody
PKB ALPHA antibody
PKB antibody
PKB beta antibody
PKB gamma antibody
PKB-GAMMA antibody
PKB/Akt antibody
PKBALPHA antibody
PKBB antibody
PKBBETA antibody
PKBG antibody
PKBGAMMA antibody
PRKBA antibody
PRKBB antibody
PRKBG antibody
Protein kinase Akt 2 antibody
Protein kinase Akt-3 antibody
Protein kinase B alpha antibody
Protein kinase B antibody
Protein kinase B beta antibody
Protein kinase B gamma antibody
Proto oncogene c Akt antibody
RAC ALPHA antibody
RAC alpha serine/threonine protein kinase antibody
RAC antibody
RAC BETA antibody
RAC beta serine/threonine protein kinase antibody
RAC PK alpha antibody
RAC PK beta antibody
rac protein kinase alpha antibody
rac protein kinase beta antibody
RAC-gamma antibody
RAC-gamma serine/threonine-protein kinase antibody
RAC-PK-gamma antibody
RACALPHA antibody
RACalpha serine/threonine kinase antibody
RACBETA antibody
RACgamma antibody
RACgamma serine/threonine protein kinase antibody
RACPKgamma antibody
serine threonine protein kinase antibody
STK-2 antibody
STK2 antibody
thymoma viral proto oncogene 1 antibody
thymoma viral proto oncogene antibody
V akt murine thymoma viral oncogene homolog 1 antibody
V akt murine thymoma viral oncogene homolog 2 antibody
V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) antibody
V akt murine thymoma viral oncogene homolog 3 antibody
vakt murine thymoma viral oncogene homolog 1 antibody
vakt murine thymoma viral oncogene homolog 2 antibody
vakt murine thymoma viral oncogene homolog 3 antibody
数据库入口号Swiss-Prot#:P31749
计算分子量56 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of AKT1/2/3 on MCF-7 cell lysates using anti-AKT1/2/3 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.
ICC staining AKT1/2/3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AKT1/2/3 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AKT1/2/3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of A549 cells with AKT1/2/3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.
1. Buendia, I. et al. 2015. The Melatonin-N,N-Dibenzyl(N-methyl)amine Hybrid ITH91/IQM157 Affords Neuroprotection in an in Vitro Alzheimer's Model via Hemo-oxygenase-1 Induction. ACS chemical neuroscience. 6: 288-96.
2. Siendones, E. et al. 2014. Membrane-bound CYB5R3 is a common effector of nutritional and oxidative stress response through FOXO3a and Nrf2. Antioxidants & redox signaling. 21: 1708-25.
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