产品详情
产品名称Wilms Tumor Protein Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性SC06-41
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Hu, Ms
免疫原描述recombinant protein
别名WIT 2 antibody
WT 1 antibody
AWT1 antibody
FWT1 antibody
GUD antibody
NPHS4 antibody
WAGR antibody
Wilms tumor 1 antibody
Wilms Tumor antibody
Wilms tumor protein antibody
Wilms' tumor gene antibody
Wilms' tumor protein antibody
WIT2 antibody
WT antibody
WT1 antibody
WT1_HUMAN antibody
WT33 antibody
数据库入口号Swiss-Prot#:P19544
计算分子量55 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Wilms Tumor Protein on human kidney lysates using anti-Wilms Tumor Protein antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Wilms Tumor Protein antibody. Counter stained with hematoxylin.
ICC staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms' tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino-terminus. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.
背景文献
1. Witty AD et al. Generation of the epicardial lineage from human pluripotent stem cells. Nat Biotechnol 32:1026-35 (2014).
2. Gao Y et al. WT1 regulates the development of the posterior taste field. Development 141:2271-8 (2014).
Yes
