产品详情
产品名称DKK1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性SC06-86
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Hu, Rt
免疫原描述recombinant protein
别名Dickkopf 1 antibody
Dickkopf 1 homolog antibody
Dickkopf 1 like antibody
Dickkopf homolog 1 antibody
Dickkopf like protein 1 antibody
Dickkopf related protein 1 antibody
Dickkopf WNT signaling pathway inhibitor 1 antibody
Dickkopf-1 antibody
Dickkopf-related protein 1 antibody
DKK 1 antibody
Dkk-1 antibody
Dkk1 antibody
DKK1_HUMAN antibody
hDkk 1 antibody
hDkk-1 antibody
SK antibody
数据库入口号Swiss-Prot#:O94907
计算分子量38 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:500
FC: 1:50-1:100
Western blot analysis of DKK1 on MCF-7 cells lysates using anti-DKK1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-DKK1 antibody. Counter stained with hematoxylin.
ICC staining DKK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DKK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DKK1 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with DKK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
The Wnt genes are a group of well conserved, cysteine-rich secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the seven membrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation, causing the accumulation of b-catenin and subsequent regulation of developmentally significant Wnt |target genes. The Dickkopf family of secreted inhibitors of Wnt signaling ensures proper morphological development by antagonizing different stages of the Wnt cascade. Dkk-1 (Dickkopf-1) acts upstream of b-catenin and dishevelled to inhibit Wnt signaling. Dkk-1 is a 266-amino acid (human), secreted protein that contains a 31-residue N-terminal signal peptide, 2 cysteine rich domains, and a putative carboxy terminal N-glycosylation site. Human Dkk-1 transcripts are abundantly present in fetal kidney, adult placenta and adult prostate. Putative cis regulatory elements upstream of the Dkk-1 start site include p53, Sp1, MyoD, STAT, Oct-1/2, C/EBP-a, C/EBP-b, GATA-1, GATA-2 and GATA-3.
背景文献
1. Li Y et al. Minocycline protects against hepatic ischemia/reperfusion injury in a rat model. Biomed Rep 3:19-24 (2015).
2. Chen C et al. Elevated levels of Dickkopf-1 are associated with ?-catenin accumulation and poor prognosis in patients with chondrosarcoma. PLoS One 9:e105414 (2014).
Yes
