产品详情
产品名称WASF2 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性SC66-05
纯化ProA affinity purified
应用WB, IHC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名DICTYOSTELIUM antibody dJ393P12.2 antibody IMD2 antibody Protein WAVE-2 antibody Putative Wiskott Aldrich syndrome protein family member 4 antibody SCAR 2 antibody SCAR antibody SCAR2 antibody Suppressor of cyclic-AMP receptor (WASP family) antibody Verprolin homology domain-containing protein 2 antibody WASF2 antibody WASF2_HUMAN antibody WASF4 antibody WASP family protein member 2 antibody WASP family protein member 4 antibody WASP family Verprolin homologous protein 2 antibody WASP-family protein member 2 antibody WAVE 2 antibody WAVE2 antibody Wiskott-Aldrich syndrome protein family member 2 antibody Wiskott-Aldrich syndrome protein family verprolin-homologous protein antibody
数据库入口号Swiss-Prot#:Q9Y6W5
计算分子量54 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
Western blot analysis of WASF2 on human placenta lysates using anti-WASF2 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-WASF2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-WASF2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-WASF2 antibody. Counter stained with hematoxylin.
背景
WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in Actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the Actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating Actin reorganization downstream of the Rho family of small GTPases. The protein homologs WAVE1 and WAVE2 regulate membrane ruffling by inducing the formation of Actin filament clusters in response to GTP binding and by activating Rac. They mediate Actin polymerization by cooperating with the Arp2/3 complex, thereby promoting the formation of Actin filaments. WAVE1, which is also designated SCAR (suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed, with the expression highest in peripheral blood leukocytes. WAVE3 forms a multiprotein complex that links receptor kinases with Actin and plays a role in the transduction of signals involving changes in cell shape, function or motility.
背景文献
1. Jia, S. et al. 2014. Down-regulation of WAVE2, WASP family verprolin-homologous protein 2, in gastric cancer indicates lymph node metastasis and cell migration. Anticancer research. 34: 2185-94. 2. Goh, WI. et al. 2012. mDia1 and WAVE2 proteins interact directly with IRSp53 in filopodia and are involved in filopodium formation. J. Biol. Chem. 287: 4702-4714.