产品详情
产品名称GAP43 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性SC60-06
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名Axonal membrane protein GAP 43 antibody Axonal membrane protein GAP-43 antibody B 50 antibody Calmodulin binding protein P 57 antibody F1 antibody GAP 43 antibody GAP43 antibody Growth Associated Protein 43 antibody Growth-associated protein 43 antibody Nerve Growth Related Peptide antibody Nerve growth related peptide GAP43 antibody NEUM_HUMAN antibody Neural phosphoprotein B 50 antibody Neural phosphoprotein B-50 antibody Neuromodulin antibody Neuron growth associated protein 43 antibody PP46 antibody Protein F1 antibody QtrA-11580 antibody QtrA-13071 antibody
数据库入口号Swiss-Prot#:P17677
计算分子量48 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:2,000-1:5,000
IHC: 1:100-1:500
ICC: 1:100-1:500
FC: 1:50-1:100
Western blot analysis of GAP43 on mouse brain lysates using anti-GAP43 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GAP43 antibody. Counter stained with hematoxylin.
ICC staining GAP43 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GAP43 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY-5y cells with GAP43 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
GAP-43 (growth associated protein 43, B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1 is a crucial component for regenerative response in the nervous system. It is present at high levels in neuronal growth cones during development and axonal regeneration. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The neuron-specific Elav/Hu family member, HuD, interacts with and stabilizes GAP-43 mRNA in developing neurons and leads to increased levels of GAP-43 protein. Heterozygous GAP-43 knockout mice with GAP-43 levels reduced by one-half dipslay significant memory impairments in cued conditioning or on tests of nociceptive or auditory perception.
背景文献
1. D'Agostino G et al. Prolyl endopeptidase-deficient mice have reduced synaptic spine density in the CA1 region of the hippocampus, impaired LTP, and spatial learning and memory. Cereb Cortex 23:2007-14 (2013). 2. Figueroa JD et al. Metabolomics uncovers dietary omega-3 fatty acid-derived metabolites implicated in anti-nociceptive responses after experimental spinal cord injury. Neuroscience 255C:1-18 (2013).
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et al,Maf1 is an intrinsic suppressor against spontaneous neural repair and functional recovery after ischemic strokeInJ Adv ResOn2023 SepbyChi Kwan Tsang , Qiongjie Mi et al..PMID:36402285,
, (2023),
PMID: 36402285
et al,Maf1 is an intrinsic suppressor against spontaneous neural repair and functional recovery after ischemic stroke. In J Adv Res on 2022 Nov 17 by Chi Kwan Tsang, Qiongjie Mi,et al..PMID:36402285,
, (2022),
PMID: 36402285
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