产品详情
产品名称BRG1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性SN20-03
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名ATP dependent helicase SMARCA4 antibody ATP-dependent helicase SMARCA4 antibody BAF 190 antibody BAF190 antibody BAF190A antibody Brahma protein like 1 antibody BRG1 antibody BRG1 associated factor 190A antibody BRG1 protein antibody BRG1-associated factor 190A antibody BRM/SWI2 related gene 1 antibody Global transcription activator homologous sequence antibody global transcription activator snf2l4 antibody Homeotic gene regulator antibody hSNF2b antibody Mitotic growth and transcription activator antibody MRD16 antibody Nuclear protein GRB1 antibody Protein brahma homolog 1 antibody Protein BRG-1 antibody Protein BRG1 antibody RTPS2 antibody SMARC A4 antibody SMARCA4 antibody SMCA4_HUMAN antibody SNF2 antibody SNF2 beta antibody SNF2 like 4 antibody SNF2-beta antibody SNF2B antibody SNF2L4 antibody SNF2LB antibody Sucrose nonfermenting like 4 antibody SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 antibody SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 antibody SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 antibody SWI2 antibody Transcription activator BRG1 antibody
数据库入口号Swiss-Prot#:P51532
计算分子量185 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: HepG2
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
ICC staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
背景文献
1. Madsen MS et al. Peroxisome proliferator-activated receptor ? and C/EBPa synergistically activate key metabolic adipocyte genes by assisted loading. Mol Cell Biol 34:939-54 (2014). 2. Ramos P et al. Small cell carcinoma of the ovary, hypercalcemic type, displays frequent inactivating germline and somatic mutations in SMARCA4. Nat Genet 46:427-9 (2014).
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et al,Autoantibody against Tumor-Associated Antigens as Diagnostic Biomarkers in Hispanic Patients with Hepatocellular Carcinoma. In Cells on 2022 Oct 14 by Yangcheng Ma, Cuipeng Qiu, et al..PMID:36291095,
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PMID: 36291095
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