产品详情
产品名称PAX8 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性SD0832
纯化ProA affinity purified
应用ICC/IF, IHC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名OTTHUMP00000158659 antibody OTTHUMP00000158660 antibody OTTHUMP00000203723 antibody OTTHUMP00000203724 antibody Paired box 8 antibody Paired box gene 8 antibody paired box homeotic gene 8 antibody Paired box protein Pax 8 antibody Paired box protein Pax-8 antibody Paired domain gene 8 antibody PAX 8 antibody PAX8 antibody PAX8_HUMAN antibody
数据库入口号Swiss-Prot#:Q06710
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
IHC: 1:50-1:200
ICC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-PAX8 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PAX8 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PAX8 antibody. Counter stained with hematoxylin.
ICC staining PAX8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
The Pax family encodes transcription factors that function during embryogenesis and regulate the temporal and position-dependent differentiation of cells. Pax-8 is expressed in the developing and adult thyroid, the developing secretory system and at lower levels, in the adult kidney. Pax-8 complexes with TTF-1 and TTF-2 to induce thyroid follicular cell differentiation and thyroid hormone biosynthesis by regulating the expression of sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG) and the thyrotropin receptor (TSHR). Treatment of FRTL-5 cells with TGFβ1 decreases Pax-8 mRNA levels and Pax-8 DNA binding activity, which suppresses the expression of TG and the formation of thyrocytes. Patients who have autosomal dominant mutations of the Pax-8 gene develop thyroid dysgenesis. The Pax-8 gene produces six isoforms, A to F, that are generated by alternative splicing and differ in their carboxy-terminal regions. The Pax-8 isoforms display different DNA binding capacities and are thought to be functionally distinct. The gene which encodes Pax-8 maps to human chromosome 2q12-q14.
背景文献
1. Sicking EM et al. Subtotal ablation of parietal epithelial cells induces crescent formation. J Am Soc Nephrol 23:629-40 (2012). 2. Borgogni E et al. Elocalcitol inhibits inflammatory responses in human thyroid cells and T cells. Endocrinology 149:3626-34 (2008).
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