产品详情
产品名称Parkin Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性JF82-09
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名AR JP antibody E3 ubiquitin ligase antibody E3 ubiquitin protein ligase parkin antibody E3 ubiquitin-protein ligase parkin antibody FRA6E antibody LPRS 2 antibody LPRS2 antibody PARK 2 antibody Park2 antibody Parkin 2 antibody Parkinson disease (autosomal recessive juvenile) 2 antibody Parkinson disease (autosomal recessive, juvenile) 2, parkin antibody Parkinson disease protein 2 antibody Parkinson juvenile disease protein 2 antibody Parkinson protein 2 E3 ubiquitin protein ligase antibody Parkinson protein 2, E3 ubiquitin protein ligase (parkin) antibody PDJ antibody PRKN 2 antibody PRKN antibody PRKN2 antibody PRKN2_HUMAN antibody Ubiquitin E3 ligase PRKN antibody
数据库入口号Swiss-Prot#:O60260
计算分子量52 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:50
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Parkin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Parkin antibody. Counter stained with hematoxylin.
ICC staining Parkin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Parkin in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Parkin in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY-5Y cells with Parkin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
背景
Parkin is a zinc-finger protein that is related to ubiquitin at the amino terminus. The wild type Parkin gene, which maps to human chromosome 6q25.2-27, encodes a 465 amino acid full-length protein that is expressed as multiple isoforms. Mutations in the Parkin gene are responsible for autosomal recessive juvenile Parkinson's disease and commonly involve deletions of exons 3-5. In humans, Parkin is expressed in a subset of cells of the basal ganglia, midbrain, cerebellum and cerebral cortex, and is subject to alternative splicing in different tissues. Parkin expression is also high in the brainstem of mice, with the majority of immunopositive cells being neurons. The Parkin gene has been identified in a diverse group of organisms including mammals, birds, frog and fruit flies, suggesting that analogous functional roles of the Parkin protein may have been highly conserved during the course of evolution.
背景文献
1. Lu Y et al. Beneficial effects of astragaloside IV against angiotensin II-induced mitochondrial dysfunction in rat vascular smooth muscle cells. Int J Mol Med 36:1223-32 (2015). 2. Seillier M et al. Defects in mitophagy promote redox-driven metabolic syndrome in the absence of TP53INP1. EMBO Mol Med 7:802-18 (2015).
Yes
