产品详情
产品名称FOXA1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性JF10-02
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名forkhead box A1 antibody Forkhead box protein A1 antibody FOX A1 antibody FOXA1 antibody FOXA1_HUMAN antibody hepatocyte nuclear factor 3 alpha antibody Hepatocyte nuclear factor 3-alpha antibody HNF 3A antibody HNF-3-alpha antibody HNF-3A antibody HNF3A antibody MGC33105 antibody TCF 3A antibody TCF-3A antibody TCF3A antibody Transcription factor 3A antibody
数据库入口号Swiss-Prot#:P55317
计算分子量49 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of FOXA1 on different lysates using anti-FOXA1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: Mouse lung
Immunohistochemical analysis of paraffin-embedded rat prostate tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXA1 antibody. Counter stained with hematoxylin.
ICC staining FOXA1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homoeprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3α, HNF-3β and HNF-3γ regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3α and HNF-3β have also been shown to be involved in gastrulation events such as body axis formation. HNF-4α and HNF-4γ have been shown to be important for early embryo development. HNF-4α is expressed in liver, kidney, pancreas, small intestine, testis and colon; HNF-4γ is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3β, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and α-1 antitrypsin.
背景文献
1. Yang YA et al. FOXA1 potentiates lineage-specific enhancer activation through modulating TET1 expression and function. Nucleic Acids Res 44:8153-64 (2016). 2. Guaraldo M et al. Characterization of human mitochondrial ferritin promoter: identification of transcription factors and evidences of epigenetic control. Sci Rep 6:33432 (2016).
Yes
