产品详情
产品名称JAK3 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性JM66-31
纯化ProA affinity purified
应用WB, IHC, ICC
种属反应性Hu
免疫原描述Recombinant protein
别名EC 2.7.10.2 antibody
JAK 3 antibody
JAK L antibody
JAK-3 antibody
Jak3 antibody
JAK3 HUMAN antibody
JAK3_HUMAN antibody
JAKL antibody
Janus kinase 3 (a protein tyrosine kinase, leukocyte) antibody
Janus kinase 3 antibody
Janus Kinase3 antibody
L JAK antibody
L-JAK antibody
Leukocyte janus kinase antibody
LJAK antibody
Protein tyrosine kinase leukocyte antibody
Tyrosine protein kinase JAK3 antibody
Tyrosine-protein kinase JAK3 antibody
数据库入口号Swiss-Prot#:P52333
计算分子量125 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-JAK3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-JAK3 antibody. Counter stained with hematoxylin.
ICC staining JAK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3.
背景文献
1. Kaur M et al. Oxindole-based SYK and JAK3 dual inhibitors for rheumatoid arthritis: designing, synthesis and biological evaluation.Future Med Chem 9(11):1193-1211 (2017).
2. Shim YJ et al. Identification of Novel JAK3 Mutations by Whole-Exome Sequencing in a Korean Boy With Severe Combined Immunodeficiency.J Investig Allergol Clin Immunol 27(3):187-189 (2017).