产品详情
产品名称JAK1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性JM75-03
纯化ProA affinity purified
应用WB, IHC, FC
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein
别名JAK 1 antibody
JAK 1A antibody
JAK 1B antibody
JAK-1 antibody
JAK1 antibody
JAK1_HUMAN antibody
JAK1A antibody
JAK1B antibody
Janus kinase 1 (a protein tyrosine kinase) antibody
Janus kinase 1 antibody
JTK3 antibody
Tyrosine protein kinase JAK 1 antibody
Tyrosine protein kinase JAK1 antibody
Tyrosine-protein kinase JAK1 antibody
数据库入口号Swiss-Prot#:P23458
计算分子量133 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50
FC: 1:50-1:100
Western blot analysis of JAK1 on Hela cell using anti-JAK1 antibody at 1/1,000 dilution.
Positive control: Lane 1: PC-12 Lane 2: Jurkat Lane 3: NIH-3T3
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-JAK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-JAK1 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of SW480 cells with JAK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
背景
JAK1 (Janus kinase 1) belongs to the family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK1. JAK1 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. Upon ligand binding, JAK1 undergoes tyrosine phosphorylation and catalytic activation in an interdependent manner. Phosphorylation of tyrosine residues at position 1022 and 1023 is believed to function in the activation of catalytic events. The canonical JAK-Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Altering JAK-Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.
背景文献
1. Liu W et al. Epigallocatechin-3-gallate ameliorates seawater aspiration-induced acute lung injury via regulating inflammatory cytokines and inhibiting JAK/STAT1 pathway in rats. Mediators Inflamm 2014:612593 (2014).
2. El Jamal SM et al. Interferon gamma-induced apoptosis of head and neck squamous cell carcinoma is connected to indoleamine-2,3-dioxygenase via mitochondrial and ER stress-associated pathways. Cell Div 11:11 (2016).