产品详情
产品名称WASL Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性JU07-31
纯化ProA affinity purified
应用WB,ICC,IHC,FC
种属反应性Hu, Ms
免疫原描述Recombinant protein
别名WASP family, verprolin homology domain-containing protein 1 antibody
WASP family member 1 antibody
FLJ31482 antibody
homology of dictyostelium scar 1 antibody
KIAA0269 antibody
Protein WAVE-1 antibodyv Protein WAVE1 antibody
scar, Dictyostelium, homology of, 1 antibody
SCAR1 antibody
Similar to a plant extensin like protein antibody
Verprolin homology domain containing protein 1 antibody
Verprolin homology domain-containing protein 1 antibody
WAS protein family, member 1 antibody
Wasf1 antibody
WASF1_HUMAN antibody
WASL antibody
WASP family 1 antibody
WASP family protein member 1 antibody
WASP family, verprolin homology domain-containing protein antibody
WAVE antibody
WAVE1 antibody
Wiskott Aldrich syndrome protein family member 1 antibody
Wiskott-Aldrich syndrome protein family member 1 antibody
数据库入口号Swiss-Prot#:O00401
计算分子量55 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of WASL on mouse Human serum lysates using anti-Ubiquitin antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-WASL antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-WASL antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-WASL antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-WASL antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-WASL antibody. Counter stained with hematoxylin.
ICC staining WASL in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining WASL in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of K562 cells with WASL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
背景
This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression..
背景文献
1. Zhang Y et al. The small GTPase CDC42 regulates actin dynamics during porcine oocyte maturation. J Reprod Dev 63:505-510 (2017).
2. Bendris N et al. SNX9 promotes metastasis by enhancing cancer cell invasion via differential regulation of RhoGTPases. Mol Biol Cell pii: mbc.E16-02-0101 (2016).
Yes
