产品详情
产品名称WNK1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性JG36-91
纯化ProA affinity purified
应用WB,IHC
种属反应性Hu, Ms, Rt
免疫原描述Synthetic peptide within Human WNK1 aa 100-200 (Cysteine residue).
别名Erythrocyte 65 kDa protein antibody
HSAN2 antibody
HSN2 antibody
hWNK1 antibody
KDP antibody
KIAA0344 antibody
Kinase deficient protein antibody
MGC163339 antibody
MGC163341 antibody
p65 antibody
PPP1R167 antibody
PRKWNK1 antibody
Prostate derived sterile 20 like kinase antibody
Protein kinase lysine deficient 1 antibody
Protein kinase lysine-deficient 1 antibody
Protein kinase with no lysine 1 antibody
Protein phosphatase 1, regulatory subunit 167 antibody
PSK antibody
Serine/threonine protein kinase WNK1 1 antibody
Serine/threonine protein kinase WNK1 2 antibody
Serine/threonine protein kinase WNK1 antibody
Serine/threonine-protein kinase WNK1 antibody
With no K antibody
WNK lysine deficient protein kinase 1 antibody
WNK lysine deficient protein kinase 1 isoform antibody
WNK1 antibody
WNK1_HUMAN antibody
数据库入口号Swiss-Prot#:Q9H4A3
计算分子量250/300 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:50-1:200
Western blot analysis of WNK1 on A549 cell lysates using anti-WNK1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-WNK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-WNK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-WNK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-WNK1 antibody. Counter stained with hematoxylin.
背景
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
背景文献
1. Moore T M et al. PSK, a novel STE20-like kinase derived from prostatic carcinoma that activates the JNK MAPK pathway and regulates actin cytoskeletal organisation. J Biol Chem 275:4311-4322 (2000).
2. Lafreniere R G et al. Identification of a novel gene (HSN2) causing hereditary sensory and autonomic neuropathy type II through the study of Canadian genetic isolates. Am J Hum Genet 74:1064-1073 (2004).
Yes
