产品详情
产品名称Xanthine Oxidase Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆性JG38-40
纯化ProA affinity purified
应用WB,IHC
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein within human Xanthine Oxidase aa 100-300.
别名Xanthine dehydrogenase antibody
Xanthine dehydrogenase/oxidase antibody
Xanthine oxidase antibody
Xanthine oxidoreductase antibody
XD antibody
XDH antibody
XDH_HUMAN antibody
xdha antibody
XO antibody
xor antibody
数据库入口号Swiss-Prot#:P47989
计算分子量146 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:50-1:200
Western blot analysis of Xanthine Oxidase on human kidney tissue lysate using anti-Xanthine Oxidase antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Xanthine Oxidase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Xanthine Oxidase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Xanthine Oxidase antibody. Counter stained with hematoxylin.
背景
The process of metabolizing purines to a common molecule known as xanthine is an essential process for the proper shuttling of uric acid. Xanthine oxidase is a flavoprotein enzyme that coordinates molybdenum and utilizes NAD+ as an electron acceptor to catalyze the oxidation of hypoxanthine to xanthine and then to uric acid. The predominant form of this enzyme is xanthine dehydrogenase, which is a homodimer that can be converted to xanthine oxidase by sulfhydryl oxidation or proteolytic modification. Xanthine oxidase is present in species ranging from bacteria to human and is ubiquitously expressed in mammalian tissues. In the oxidase form, this enzyme is coupled to the generation of free radicals. Individuals showing marked elevation of serum xanthine oxidase is suggestive of chronic liver disease and cholestasis, which is a condition defined by hepatic obstruction. Hepatic obstruction causes bile salts, the bile pigment bilirubin, and fats to accumulate in the blood stream instead of being eliminated normally. The clinical consequences of defects in xanthine oxidase range from mild to severe and even contribute to fatal disorders.
背景文献
1. Yamaguchi Y et al. Human xanthine oxidase changes its substrate specificity to aldehyde oxidase type upon mutation of amino acid residues in the active site: roles of active site residues in binding and activation of purine substrate. J Biochem 141:513-524 (2007).
2. Saksela M et al. Cloning and expression in vitro of human xanthine dehydrogenase/oxidase. Biochem J 315:235-239 (1996).