Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LY9 in samples. An antibody specific for LY9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLY9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LY9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LY9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Lymphocyte antigen 9, also known as LY9, has also recently been designated CD229 . LY9 has been shown to interact with SH2D1A.By sequence analysis of cDNA clones, Sandrin et al. (1992) demonstrated that the mouse lymphocyte surface antigen Ly9 is a novel member of a subgroup of the immunoglobulin superfamily, together with the antigens CD2, BCM1 (CD48), and LFA3 (CD58). Kingsmore et al. (1995) noted that, though the function of Ly9 was unknown, other subgroup members are involved in adhesion reactions between T lymphocytes and accessory cells. CD2, CD48, and CD58 are also distinguished by homophilic interactions with one another; thus, human CD48 and CD58 are both ligands for CD2.