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位置: 首页 > 磷酸化抗体 > AFX(Phospho-Ser197) Antibody

AFX(Phospho-Ser197) Antibody#11137

AFX(Phospho-Ser197) Antibody
AFX(Phospho-Ser197) Antibody
AFX(Phospho-Ser197) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称AFX(Phospho-Ser197) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of AFX only when phosphorylated at serine 197.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human AFX.

基因/蛋白名称AFX

修饰Phospho

别名AFX; FOXO4; AFX1; Afxh;

数据库入口号Swiss-Prot: P98177
NCBI Protein: NP_001164402.1

UniprotP98177

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 65kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells untreated or treated with serum using AFX(Phospho-Ser197) Antibody #11137.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX(Phospho-Ser197) Antibody #11137(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using AFX(Phospho-Ser197) Antibody #11137.
背景
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.

Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
Essers MA, et al. EMBO J 2004 Nov. 11.
Brownawell AM, (2001) Mol Cell Biol; 21(10): 3534-46.
Kops GJ, et al. (1999) Nature; 398(6728): 630-4.

如果您使用该产品11137发表了文章,请通知我们,让我们可以引用您的文献。

et al,Activation of Akt/FoxO and inactivation of MEK/ERK pathways contribute to induction of neuroprotection against transient global cerebral ischemia by delayed hypoxic postconditioning in adult rats.In Neuropharmacology on 2012 Oct by Zhan L, Li D,et al..PMID:22749925, , (2012),
PMID: 22749925
et al,Activation of Akt/FoxO signaling pathway contributes to induction of neuroprotection against transient global cerebral ischemia by hypoxic pre-conditioning in adult rats.In J Neurochem on 2010 Aug by Zhan L, Wang T, et al..PMID:20492357, , (2010),
PMID: 20492357
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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