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位置: 首页 > 磷酸化抗体 > eNOS(Phospho-Ser1177) Antibody

eNOS(Phospho-Ser1177) Antibody#11156

eNOS(Phospho-Ser1177) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称eNOS(Phospho-Ser1177) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of eNOS only when phosphorylated at serine 1177.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 1177 (T-Q-S(p)-F-S) derived from Human eNOS.

基因/蛋白名称eNOS

修饰Phospho

别名Constitutive NOS; EC-NOS; ECNOS; NOS3; NOSIII

数据库入口号Swiss-Prot: P29474
NCBI Protein: NP_000594.2

UniprotP29474

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 133kd

Western blotting: 1:500~1:1000

Western blot analysis of extracts from 3T3 cells untreated and treated with EGF, HUVEC cells treated with VEGF, JK cells untreated and treated with serum using eNOS (Phospho-Ser1177) Antibody #11156.
背景

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Fulton, D. et al. (1999) Nature 399, 597-601.
Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

如果您使用该产品11156发表了文章,请通知我们,让我们可以引用您的文献。

et al,Adenosine mono-phosphate-activated protein kinase-mammalian target of rapamycin signaling participates in the protective effect of chronic intermittent hypobaric hypoxia on vascular endothelium of metabolic syndrome rats. In Chin J Physiol on 2022 Mar-Apr by Fang Cui, Min Shi,et al..PMID:35488670, , (2022),
PMID: 35488670
et al,Chaiqi decoction ameliorates vascular endothelial injury in metabolic syndrome by upregulating autophagy. In Am J Transl Res on 2020 Sep 15 by Xun Chen, Xiao-Ru Yan,et al..PMID:33042397, , (2020),
PMID: 33042397
et al,Chronic intermittent hypobaric hypoxia protects vascular endothelium by ameliorating autophagy in metabolic syndrome rats. In Life Sci.On 2018 Jul 15 by Cui F, Guan Y et al..PMID:29733850, , (2018),
PMID: 29733850
et al,Pro-atherosclerotic disturbed flow disrupts caveolin-1 expression, localization, and function via glycocalyx degradation.In J Transl Med. On 2018 Dec 18 by Harding IC, Mitra R et al..PMID: 30563532 , , (2018),
PMID: 30563532
et al,Gardenamide A protects RGC-5 cells from H2O2-induced oxidative stress insults by activating PI3K/Akt/eNOS signaling pathway.In Int J Mol Sci on 2015 Sep 15 by Rikang Wang , Lizhi Peng et al..PMID:26389892, , (2015),
PMID: 26389892
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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