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位置: 首页 > 磷酸化抗体 > eNOS (Phospho-Ser114) Antibody

eNOS (Phospho-Ser114) Antibody#12472

eNOS (Phospho-Ser114) Antibody
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产品总价:
产品详情

产品名称eNOS (Phospho-Ser114) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性eNOS (Phospho-Ser114) Antibody detects endogenous levels of eNOS only when phosphorylated at Ser114

免疫原类型Peptide

免疫原描述A synthesized peptide derived from human eNOS (Phospho-Ser114)

基因/蛋白名称eNOS

修饰Phospho

别名NOS3, Constitutive NOS, CNOS, Endothelial NOS, ECNOS, NOS type III, NOSIII, EC-NOS, ENOS, Nitric oxide synthase 3

数据库入口号Swiss-Prot#: P29474
NCBI Gene ID: 4846

UniprotP29474

目标蛋白种属human

计算分子量140kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:1000

Western Blot analysis of lysates of HEK-293 cell (Left) and HEK-293 cell treated with 100ng/mL LPS for 30min (Right), using primary antibody at 1:1000 dilution.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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