产品详情
产品名称PARP1 Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC,IF
种属反应性Human,Mouse,Rat
免疫原描述Recombinant fusion protein of human PARP1 (NP_001609.2).
别名PARP1; ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PPOL; pADPRT-1; poly(ADP-ribose) polymerase 1
数据库入口号Swiss-Prot#:P09874
NCBI Gene ID:142
计算分子量89kDa, 116kDa
配方Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
保存Store at -20˚C
应用详情
WB 1:1000 - 1:3000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:50 - 1:200
Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using PARP1 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using PARP1 antibody at dilution of 1:200 (40x lens).
Confocal immunofluorescence analysis of U-2 OS cells using PARP1 Polyclonal antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of Jurkat cells, using PARP1 antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines, using PARP1 antibody at 1:500 dilution.
Western blot analysis of extracts from normal (control) and PARP1 knockout (KO) HeLa cells, using PARP1 antibody at 1:1000 dilution.
Immunofluorescence analysis of U2OS cells using PARP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
背景
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
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et al,CD155 Cooperates with PD-1/PD-L1 to Promote Proliferation of Esophageal Squamous Cancer Cells via PI3K/Akt and MAPK Signaling Pathways. In Cancers (Basel) on 2022 Nov 15 by Xiyang Tan, Jie Yang, et al..PMID:36428703,
, (2022),
PMID: 36428703
货期一周
