免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > PKCth(Phospho-Ser695) Antibody

PKCth(Phospho-Ser695) Antibody#11173

PKCth(Phospho-Ser695) Antibody
PKCth(Phospho-Ser695) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称PKCth(Phospho-Ser695) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of PKCth only when phosphorylated at serine 695.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 695 (N-F-S(p)-F-M) derived from Human PKCth.

基因/蛋白名称PKCth

修饰Phospho

别名KPCT; PKC-theta; PKCQ; PRKCQ; PRKCT

数据库入口号Swiss-Prot: Q04759
NCBI Protein: NP_006248.1

UniprotQ04759

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 80kd
Western blotting: 1:500
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells untreated or treated with PMA using PKCth(Phospho-Ser695) Antibody #11173.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Phospho-Ser695) Antibody #11173(left) or the same antibody preincubated with blocking peptide(right).
背景
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Xu ZB, et al.(2004) J Biol Chem 279:50401-50409
Thebault S, et al. (2004) Mol Immunol 40: 931-942

如果您使用该产品11173发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com