产品详情
产品名称ATP6V1E1 Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC,IF
种属反应性Human,Mouse,Rat
免疫原描述Recombinant fusion protein of human ATP6V1E1 (NP_001687.1).
别名ATP6V1E1;ATP6E;ATP6E2;ATP6V1E;P31;Vma4;ARCL2C
数据库入口号Uniprot:P36543
GeneID:529
UniprotP36543
Gene ID529
计算分子量35kDa
实际分子量31KDa
配方PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody.
Western blot analysis of extracts of various cell lines, using ATP6V1E1 antibody.
Immunofluorescence analysis of HeLa cells using ATP6V1E1 Rabbit pAb.
背景
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome.