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位置: 首页 > 磷酸化抗体 > Raf1(Phospho-Ser338) Antibody

Raf1(Phospho-Ser338) Antibody#11204

Raf1(Phospho-Ser338) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Raf1(Phospho-Ser338) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Raf1 only when phosphorylated at serine 338.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 338 (R-D-S(p)-S-Y) derived from Human RAF.

基因/蛋白名称Raf1

修饰Phospho

别名C-RAF; C-Raf; CRAF

数据库入口号Swiss-Prot: P04049
NCBI Protein: NP _002871.1

UniprotP04049

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 73kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from MDA cells untreated or treated with PMA for the indicated times using Raf1(Phospho-Ser338) Antibody #11204.
背景
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

Adams DG,et al. (2005)J Biol Chem. 280(52):42644-54.
Miura S, et al. (2003) Biochem Biophys Res Commun. 306(4):924-9.
Noser JA,et al.(2007)Mol Ther.15(8):1531-6.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com