• Aluminium pouches with a Microwell Plate coated with antibody to human IL-3 (8x12)
• 2 vials human IL-3 Standard lyophilized, 1000 pg/ml upon reconstitution
• 2 vials concentrated Biotin-Conjugate anti-human IL-3 antibody
• 2 vials Streptavidin-HRP solution
• 1 bottle Standard /sample Diluent
• 1 bottle Biotin-Conjugate antibody Diluent
• 1 bottle Streptavidin-HRP Diluent
• 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
• 1 vial Substrate Solution
• 1 vial Stop Solution
• 4 pieces Adhesive Films
• package insert

Interleukin-3, also known as mast cell growth factor, P-cell stimulating factor, burst promoting activity, multicolony stimulating factor, t h y-1 inducing factor and WEHI-3 growth factor, is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. Mature human IL-3 share only 29% aa sequence identity with murine IL-3. IL-3 activity is highly speciesspecific and human IL-3 does not show activity on murine cells. IL-3 is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

Mach, N. et al. (1998) Blood 91:778.

Lantz, C.S. et al. (1998) Nature 392:90.

Kitamura, T and Miyajima, A. (1992) Blood 80:84.

Muli, A. L. et al. (1996) EMBO J 145:2425.

Lopez, A. F. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11842.