产品详情
产品名称MEK1/MEK2(Phospho-Ser217/Ser221) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用IHC WB
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of
MEK1/MEK2 only when phosphorylated at serine 217/221.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 217/221 (I-D-S(p)-M-A) derived from Human MEK1/MEK2.
基因/蛋白名称MEK1/MEK2
修饰Phospho
别名ERK activator kinase 1/ERK activator kinase2; MAP kinase kinase 1/MAP kinase kinase 2; MAP2K1/MAP2K1; MAPK/ERK kinase 1/MAPK/ERK kinase 2; MAPKK 1/MAPKK 1
数据库入口号Swiss-Prot: Q02750
NCBI Protein: NP _002746.1 NP _109587.1
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 45kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
背景
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Shen R, et al. (2002) Mol Cell Biol; 22(10): 3230-3236
Preisinger C, et al. (2005) EMBO J; 24(4): 753-765
Laine P, et al. (2000) Biochem J; 349(Pt 1): 19-25
Yaglom J, et al. (2003) Mol Cell Biol; 23(11): 3813-3824
Dahan S, et al. (2002) Infect Immun; 70(5): 2304-2310
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