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位置: 首页 > ELISA试剂盒 > Human MCP-1 ELISA kit

Human MCP-1 ELISA kit#EK0488

Human MCP-1 ELISA kit
是否有货: 1-2周
产品总价:
产品详情

产品名称Human MCP-1 ELISA kit

简述ELISA Kit

应用ELISA

种属反应性Hu

特异性Natural and recombinant Human MCP-1 Ligand

交叉反应性No significant interference observed with available related molecules.

基因/蛋白名称Human MCP-1

应用详情
Detect Range: 31.25-2000pg/ml
Sensitivity: 15pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric
Representative standard curve for MCP-1 ELISA. MCP-1 was diluted in serial two-fold steps in Sample Diluent.
产品描述
  • Aluminium pouches with a Microwell Plate coated with antibody to human MCP-1 (8x12)
  • 2 vials human MCP-1 Standard lyophilized, 1000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human MCP-1 antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
背景

Monocyte chemoattractant protein-1 (MCP-1) called also monocyte chemotactic protein-1. MCP-1 is an 8.6kDa protein and belongs to the family of chemotactic cytokines known as Chemokines . MCP-1 is expressed by monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cell, osteoblastic cells, and human pulmonary type-2-like epithelial cells in culture. MCP-1 exhibits chemotactic activity for monocytes/macrophages, basophils, T lymphocytes, particularly memory T cells and natural killer cells and neural stem cells. Elevated levels of MCP-1 are detected during inflammation and immune responses. MCP-1 activity is also important in wound healing as shown by delayed wound repair in MCP-1deficient mice. MCP-1 is implicated in the pathogenesis of disease states that involve monocyte/macrophage infiltrates, such as psoriasis, rheumatoid arthritis and recruitment of monocytes to the arterial wall in atherosclerosis. In addition to its chemotactic function, MCP-1 also induces the expression of IL-10 from macrophages, which favours a TH2 immune response. MCP-1 is a glycopeptide containing one potential N-linked glycosylation site. The extent of MCP-1 glycosylation influences its chemotactic potency and half-life in vivo.

背景文献

Boring, L.et al.( 1996). J Biol Chem.271:7551.

Capsoni, F. et al.( 1989) J Immunol Methods.120:125.

Hsu, S.M. et al. (1981). Am J Clin Pathol.75:734.

Hsu, S.M. et al. (1981)J Histochem Cytochem. 29:577.

Matsushima, K. et al. (1989)Cytokine.1:2.

Peri, G. et al. (1994)J Immunol Methods. 174:249.

Prussin, C. and Metcalfe, D.D. (1995) J Immunol Methods.188:117.

Rollins, B.J. et al.( 1989) Mol Cell Biol. 9:4687.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

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Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com