产品详情
产品名称ZP2 Rabbit Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IF
种属反应性Human,Mouse,Rat
免疫原描述Recombinant fusion protein of human ZP2 (NP_003451.1).
别名ZP2;ZPA;Zp-2
数据库入口号Swiss Prot:Q05996
GeneID:7783
UniprotQ05996
计算分子量81kDa/82kDa
实际分子量68kDa
配方Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:1000 - 1:4000
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ZP2 at 1:1000 dilution.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.
背景
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants.