产品详情
产品名称KIF14 Rabbit Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC,IF
种属反应性Human,Mouse,Rat
免疫原描述Recombinant fusion protein of human KIF14 (NP_055690.1).
别名KIF14;MKS12
数据库入口号Swiss Prot:Q15058
GeneID:9928
UniprotQ15058
计算分子量186kDa
实际分子量190kDa
配方Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Immunohistochemistry of paraffin-embedded rat ovary using KIF14 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using KIF14 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using KIF14 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using KIF14 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using KIF14 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using KIF14 at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using KIF14 at 1:1000 dilution.
Immunofluorescence analysis of L929 cells using KIF14 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using KIF14 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
背景
This gene encodes a member of the kinesin-3 superfamily of microtubule motor proteins. These proteins are involved in numerous processes including vesicle transport, chromosome segregation, mitotic spindle formation, and cytokinesis. In human HeLa-S3 and 293T cells, this protein is localized to the cytoplasm during interphase, to the spindle poles and spindle microtubules during mitosis, and to the midbody during cytokinesis. An internal motor domain displays microtubule-dependent ATPase activity, consistent with its function as a microtubule motor protein. Knockdown of this gene results in failed cytokinesis with endoreplication, which results in multinucleated cells. This gene has been identified as a likely oncogene in breast, lung and ovarian cancers, as well as retinoblastomas and gliomas. Alternative splicing results in multiple transcript variants.