Yes
Western blot analysis of extracts of various cell lines, using IRE1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using IRE1 Rabbit pAbat dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using IRE1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
