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位置: 首页 > 磷酸化抗体 > b-Catenin(Phospho-Ser33) Antibody

b-Catenin(Phospho-Ser33) Antibody#11218

b-Catenin(Phospho-Ser33) Antibody
b-Catenin(Phospho-Ser33) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称b-Catenin(Phospho-Ser33) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of b-Catenin only when phosphorylated at serine 33.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 33 (L-D-S(p)-G-I) derived from Human b-Catenin.

基因/蛋白名称b-Catenin

修饰Phospho

别名CTNNB1; CATNB; CTNB1; CTNNB;

数据库入口号Swiss-Prot: P35222
NCBI Protein: NP_001091679.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 92kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from MCF-7 cells untreated or treated with Calyculin A using b-Catenin(Phospho-Ser33) Antibody #11218.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using b-Catenin(Phospho-Ser33) Antibody #11218(left) or the same antibody preincubated with blocking peptide(right).
背景
Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

Novak A, et al. (1998) Proc Natl Acad Sci U S A; 95(8): 4374-4379
Marin O, et al. (2003) Proc Natl Acad Sci U S A; 100(18): 10193-10200
Okamura H, et al. (2004) Mol Cell Biol; 24(10): 4184-4195
Xing Y, et al. (2003) Genes Dev; 17(22): 2753-2764
Barth AI, et al. (1999) Proc Natl Acad Sci U S A; 96(9): 4947-4952

如果您使用该产品11218发表了文章,请通知我们,让我们可以引用您的文献。

et al,Effect of Small-molecule GSK3 Antagonist on Differentiation of Rat Dental Pulp Cells into Odontoblasts.In In Vivo on 2020 May-Jun by Masuda Y, Sakagami H, et al..PMID: 32354894 , , (2020),
PMID: 32354894
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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