产品详情
产品名称EGFR(Phospho-Tyr1172) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level EGFR only when phosphorylated at tyrosine 1172.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 1172 (P-D-Y(p)-Q-Q) derived from Human EGFR.
基因/蛋白名称EGFR
修饰Phospho
别名ERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR
数据库入口号Swiss-Prot: P00533
NCBI Protein: NP_005219.2
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
背景
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Noguchi T, et al. (1994) Mol Cell Biol 14(10): 6674-6682
Doherty JK, et al. (1999) Proc Natl Acad Sci U S A 96(19): 10869-10874
Kanner SB, et al. (1991) Mol Cell Biol 11(2): 713-720
Wu TT, et al. (1998) Mol Biol Cell 9(7): 1661-1674
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et al,Tumor-penetrating Peptide Fused EGFR Single-Domain Antibody Enhances Cancer Drug Penetration Into 3D Multicellular Spheroids and Facilitates Effective Gastric Cancer Therapy .In J Control Release on 2015 Feb 28 by Huizi Sha , Zhengyun Zou et al..PMID: 25553823,
, (2015),
PMID: 25553823
et al,EGFR phosphorylation of DCBLD2 recruits TRAF6 and stimulates AKT-promoted tumorigenesis. In J Clin Invest on 2014 Sep by An-Jey A Su, Philip E Auron, et al..PMID:25061874
,
, (2014),
PMID: 25061874
et al,Epidermal Growth Factor (EGF)-enhanced Vascular Cell Adhesion molecule-1 (VCAM-1) Expression Promotes Macrophage and Glioblastoma Cell Interaction and Tumor Cell Invasion.In J Biol Chem on 2013 Nov 1 by Yanhua Zheng, Weiwei Yang,et al..PMID:24045955,
, (2013),
PMID: 24045955
et al,EGF-enhanced vascular cell adhesion molecule-1 (VCAM-1) expression promotes macrophage and glioblastoma cell interaction and tumor cell invasion. In J Biol Chem on 2013 Nov 1 by Yanhua Zheng, Weiwei Yang, et al..PMID: 24045955,
, (2013),
PMID: 24045955
et al,Epidermal growth factor (EGF)-enhanced vascular cell adhesion molecule-1 (VCAM-1) expression promotes macrophage and glioblastoma cell interaction and tumor cell invasion.. In J Biol Chem on 2013 Nov 1 by Yanhua Zheng, Weiwei Yang, et al..PMID:24045955,
, (2013),
PMID: 24045955
Yang W, Zheng Y, Xia Y et al,ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect.,
Nature Cell Biology, 14(12):1295-1304 (2012),
PMID: 23178880
et al,EGFR-induced and PKC蔚 monoubiquitylation-dependent NF-魏B activation upregulates PKM2 expression and promotes tumorigenesis. In Mol Cell on 2012 Dec 14 by
Weiwei Yang, Yan Xia, et al..PMID: 23123196,
, (2012),
PMID: 23123196
查看更多
et al,PKM2 Phosphorylates Histone H3 and Promotes Gene Transcription and Tumorigenesis. In Cell on 2012 Aug 17 by Weiwei Yang, Yan Xia, et al..PMID: 22901803,
, (2012),
PMID: 22901803
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