免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > EGFR(Phospho-Tyr869) Antibody

EGFR(Phospho-Tyr869) Antibody#11229

EGFR(Phospho-Tyr869) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称EGFR(Phospho-Tyr869) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 869.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 869 (K-E-Y(p)-H-A) derived from Human EGFR.

基因/蛋白名称EGFR

修饰Phospho

别名ERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR

数据库入口号Swiss-Prot: P00533
NCBI Protein: NP_005219.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from MDA cells untreated or treated with EGF using EGFR(Phospho-Tyr869) Antibody #11229.
背景
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

Inoue A, et al. (2005) PLoS Med; 2(1): e13
Sun H, et al. (2004) EMBO J; 23(1): 100-110
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
O

如果您使用该产品11229发表了文章,请通知我们,让我们可以引用您的文献。

et al,Mutual regulation between phosphofructokinase 1 platelet isoform and VEGF promotes glioblastoma tumor growth. In Cell Death Dis on 2022 Nov 26 by Je Sun Lim, YuJie Shi, et al..PMID:36435833, , (2022),
PMID: 36435833
et al,EGFR-Phosphorylated Platelet Isoform of Phosphofructokinase 1 Promotes PI3K Activation. IN Mol Cell.On 2018 Apr 19 by Lee JH, Liu R et al..PMID:29677490, , (2018),
PMID: 29677490
et al,Secreted and O-GlcNAcylated MIF binds to the human EGF receptor and inhibits its activation.In Nat Cell Biol on 2015 Oct by Yanhua Zheng, Xinjian Li et al..PMID:26280537 , , (2015),
PMID: 26280537
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com