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位置: 首页 > 重组蛋白 > Recombinant Human DES1-3 Insulin-like Growth Factor 1

Recombinant Human DES1-3 Insulin-like Growth Factor 1#AP60063

Recombinant Human DES1-3 Insulin-like Growth Factor 1
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Human DES1-3 Insulin-like Growth Factor 1

来源种属E.coli

纯化> 97 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

内毒素Less than 50 EU/mg of rHuDES1-3 IGF-1 as determined by LAL method.

别名DES1-3 IGF-I, DES(1-3) IGF-I, IGF-I DES(1-3), DES1-3/Insulin-Like Growth factor 1

UniprotP05019

Gene ID3479

计算分子量Approximately 7.4 kDa, a single non-glycosylated polypeptide chain containing 67 amino acids.

序列TLCGAELVDA LQFVCGDRGF YFNKPTGYGS SSRRAPQTGI VDECCFRSCD LRRLEMYCAP LKPAKSA

配方Lyophilized from a 0.2 um filtered concentrated solution in PBS. 

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

背景

IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulates the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. DES(1-3)IGF-1, is a truncated variant of human IGF-1 with the tripeptide Gly-Pro-Glu absent from the N-terminus. It has been isolated from bovine colostrum, human brain and porcine uterus. The DES(1-3)IGF-1 probably results from post-translational cleavage of IGF-1. It has about 10-fold more potent than IGF-1 at stimulating hypertrophy and proliferation of cultured cells, a consequence of much reduced binding to IGF-binding proteins, in turn caused by the absence of the glutamate at position 3. Clinical opportunities for DES(1-3)IGF-1 have not yet been evaluated, but could apply in catabolic states as well as for the treatment of inflammatory bowel diseases.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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