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位置: 首页 > 磷酸化抗体 > MARCKS(Phospho-Ser162) Antibody

MARCKS(Phospho-Ser162) Antibody#11265

MARCKS(Phospho-Ser162) Antibody
MARCKS(Phospho-Ser162) Antibody
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产品总价:
产品详情

产品名称MARCKS(Phospho-Ser162) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 162 (K-K-S(p)-F-K) derived from Human MARCKS.

基因/蛋白名称MARCKS

修饰Phospho

别名MACS; MARCS; PKCSL; PRKCSL; Protein kinase C substrate

数据库入口号Swiss-Prot: P29966
NCBI Protein: NP_002347.5

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells untreated or treated with PMA using MARCKS(Phospho-Ser162) Antibody #11265.
Immunofluorescence staining of methanol-fixed Hela cells using MARCKS(Phospho-Ser162) Antibody #11265.
背景
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412
Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609
Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159

如果您使用该产品11265发表了文章,请通知我们,让我们可以引用您的文献。

et al,Phosphoproteome of signaling by ErbB2 in ovarian cancer cells. In Biochim Biophys Acta Proteins Proteom on 2022 Apr 1 by C Sidhanth, S Bindhya,et al..PMID:35158093, , (2022),
PMID: 35158093
et al,Delta Opioid Receptor Activation with Delta Opioid Peptide [d-Ala2, d-Leu5] Enkephalin Contributes to Synaptic Improvement in Rat Hippocampus against Global Ischemia. In Cell Transplant on Jan-Dec 2021 by Guangming Zhang, Zelin Lai, et al..PMID:34470528, , (2021),
PMID: 34470528
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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