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位置: 首页 > 磷酸化抗体 > p62Dok(phospho-Tyr362) Antibody

p62Dok(phospho-Tyr362) Antibody#11276

p62Dok(phospho-Tyr362) Antibody
p62Dok(phospho-Tyr362) Antibody
p62Dok(phospho-Tyr362) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称p62Dok(phospho-Tyr362) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of p62Dok only when phosphorylated at tyrosine 362.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 362 (P-I-Y(p)-D-E) derived from Human p62Dok.

基因/蛋白名称p62Dok

修饰Phospho

别名DOK1

数据库入口号Swiss-Prot: Q99704
NCBI Protein: NP_001372.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 62kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from Jurkat cells untreated or treated with insulin using p62Dok(phospho-Tyr362) Antibody #11276.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok(Phospho-Tyr362) Antibody #11276(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), using p62Dok (phospho-Tyr362) Antibody #11276.
背景

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

Zhou Songyang, et al. (2001) J. Biol. Chem ; 276: 2459 - 2465.
Jean-Guy N

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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