免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > Keratin 8(Phospho-Ser74) Antibody

Keratin 8(Phospho-Ser74) Antibody#11307

Keratin 8(Phospho-Ser74) Antibody
Keratin 8(Phospho-Ser74) Antibody
Keratin 8(Phospho-Ser74) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Keratin 8(Phospho-Ser74) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu

特异性The antibody detects endogenous level of Keratin 8 only when phosphorylated at serine 74.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 74 (L-L-S(p)-P-L) derived from Human Keratin 8 (CK8).

基因/蛋白名称Keratin 8

修饰Phospho

别名CK 8; CK8; CYK8; Cytokeratin endo A; K8

数据库入口号Swiss-Prot: P05787
NCBI Protein: NP_002264.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 55kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using Keratin 8(Phospho-Ser74) Antibody #11307.
Immunofluorescence staining of methanol-fixed Hela cells using Keratin 8(Phospho-Ser74) Antibody #11307.
Western blot analysis of extracts from 293 cells, treated with calf intestinal phosphatase (CIP), using Keratin 8 (Phospho-Ser74) Antibody #11307.
背景

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Nakamichi I, et al. (2002) Mol Biol Cell; 13(10): 3441-3451.
Prochasson P, et al. (2002) Nucleic Acids Res; 30(15): 3312-3322.
Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91(3): 873-877.
Thorey IS, et al. (1993) Mol Cell Biol; 13(11): 6742-6751

如果您使用该产品11307发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com