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位置: 首页 > 磷酸化抗体 > MAPKAPK-2(Phospho-Thr334) Antibody

MAPKAPK-2(Phospho-Thr334) Antibody#11308

MAPKAPK-2(Phospho-Thr334) Antibody
MAPKAPK-2(Phospho-Thr334) Antibody
MAPKAPK-2(Phospho-Thr334) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称MAPKAPK-2(Phospho-Thr334) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of MAPKAPK-2 only when phosphorylated at threonine 334.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 334 (P-Q-T(p)-P-L) derived from Human MAPKAPK-2.

基因/蛋白名称MAPKAPK-2

修饰Phospho

别名MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPK2; MAPKAP kinase 2; MAPKAPK-2

数据库入口号Swiss-Prot: P49137
NCBI Protein: NP_004750.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 49kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using MAPKAPK-2(Phospho-Thr334) Antibody #11308.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK-2(Phospho-Thr334) Antibody #11308(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using MAPKAPK-2(Phospho-Thr334) Antibody #11308.
背景
MAPKAPK-2 encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Rouse, J. et al. (1994) Cell 78, 1027-1037.
Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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