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位置: 首页 > 磷酸化抗体 > TrkB(Phospho-Tyr515) Antibody

TrkB(Phospho-Tyr515) Antibody#11327

TrkB(Phospho-Tyr515) Antibody
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产品总价:
产品详情

产品名称TrkB(Phospho-Tyr515) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of TrkB only when phosphorylated at tyrosine 515.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 515 (P-Q-Y(p)-F-G) derived from Human TrkB.

基因/蛋白名称TrkB

修饰Phospho

别名BDNF/NT-3 growth factors receptor precursor; GP145-TrkB; GP145-TrkB/GP95-TrkB; NTRK2; Trk-B

数据库入口号Swiss-Prot: Q16620
NCBI Protein: NP_001007098.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 140kd
Immunofluorescence: 1:100~1:200
Immunofluorescence staining of methanol-fixed Hela cells using TrkB(Phospho-Tyr515) Antibody #11327.
背景
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Woronowicz A, et al. Glycobiology. 2007 Jan;17(1):10-24.
Mojsilovic-Petrovic J, et al. J Neurosci. 2006 Sep 6;26(36):9250-63.
Lewis MA, et al. Mol Pharmacol. 2006 Apr;69(4):1396-404.
Cai D, et al. Physiol Genomics. 2006 Feb 14;24(3):191-7.

如果您使用该产品11327发表了文章,请通知我们,让我们可以引用您的文献。

YeeWen Candace Wu, Rachel A. Hill, Maren Klug et al,Sex-specific and region-specific changes in BDNF篓CTrkB signalling in the hippocampus of 5-HT1A receptor and BDNF single and double mutant mice., Brain Research., 1452:10-17 (2012),
PMID: 22464183
et al,The novel squamosamide derivative FLZ enhances BDNF/TrkB/CREB signaling and inhibits neuronal apoptosis in APP/PS1 mice.In Acta Pharmacol Sin on 2010 Mar by Li N, Liu GT et al..PMID:20154710, , (2010),
PMID: 20154710
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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