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位置: 首页 > 磷酸化抗体 > PLCg1(phospho-Tyr771) Antibody

PLCg1(phospho-Tyr771) Antibody#11523

PLCg1(phospho-Tyr771) Antibody
PLCg1(phospho-Tyr771) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称PLCg1(phospho-Tyr771) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of PLC-gamma1 only when phosphorylated at tyrosine 771.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 771 (P-D-Y(p)-G-A) derived from Human PLC-g1.

基因/蛋白名称PLCg1

修饰Phospho

别名Phosphoinositide phospholipase C; Phospholipase C-gamma-1;

数据库入口号Swiss-Prot: P19174
NCBI Protein: NP_002651.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 3T3 cells untreated or treated with serum using PLC-g1(phospho-Tyr771) Antibody #11523.
Immunofluorescence staining of methanol-fixed Hela cells using PLC-g1(phospho-Tyr771) Antibody #11523.
背景
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.

Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027.
Margolis, B. et al. (1989) Cell 57, 1101-1107.
Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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025-58868422

Email Address

techcn@signalwayantibody.com