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位置: 首页 > 磷酸化抗体 > Cdc25B(Phospho-Ser149) Antibody

Cdc25B(Phospho-Ser149) Antibody#11553

Cdc25B(Phospho-Ser149) Antibody
Cdc25B(Phospho-Ser149) Antibody
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产品总价:
产品详情

产品名称Cdc25B(Phospho-Ser149) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB

种属反应性Ms

特异性The antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B.

基因/蛋白名称Cdc25B

修饰Phospho

别名CDC25B

数据库入口号Swiss-Prot: Q9DBN8
NCBI Protein: NP_001104545.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 62kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with Hydroxyurea(lane 2) using Cdc25B(Phospho-Ser149) Antibody #11553.
Western blot analysis of extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), using Cdc25B (Phospho-Ser149) Antibody #11553.
背景

Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Li G, et al. (2010) Retrovirology.7:59.
Wang Z,et al. (2010) BMC Cancer.10:233.
Lucci MA,et al. (2010) Cell Oncol.32(5-6):361-72.

如果您使用该产品11553发表了文章,请通知我们,让我们可以引用您的文献。

Jianying Xiao, Chao Liu, Junjie Hou et al,Ser149 Is Another Potential PKA Phosphorylation Target of Cdc25B in G2/M Transition of Fertilized Mouse Eggs., THE JOURNAL OF BIOLOGICAL CHEMISTRY, 286(12):10356篓C10366 (2011),
PMID: 21212267
et al,Ser149 is another potential PKA phosphorylation target of Cdc25B in G2/M transition of fertilized mouse eggs. In J Biol Chem on 2011 Mar 25 by Jianying Xiao, Chao Liu, et al..PMID: 21212267 , , (2011),
PMID: 21212267
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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