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NFkB-p65(Ab-468) Antibody#21013

NFkB-p65(Ab-468) Antibody
NFkB-p65(Ab-468) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称NFkB-p65(Ab-468) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total NFkB-p65 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.466~470 (L-A-S-V-D) derived from Human NFkB-p65.

基因/蛋白名称NFkB-p65

别名NFKB3; RELA; TF65; Transcription factor p65; p65

数据库入口号Swiss-Prot: Q04206
NCBI Protein: NP_001138610.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 65kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
 

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p65(Ab-468) Antibody #21013(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from Heia, 293 and HT29 cells using NFκB-p65 (Ab-468) Antibody #21013.
背景

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex

Buss H, et al. (2004) J Biol Chem
Schmitz ML, et al. (2004) Blood
 

如果您使用该产品21013发表了文章,请通知我们,让我们可以引用您的文献。

et al,Cinnamaldehyde Inhibits Inflammation of Human Synoviocyte Cells Through Regulation of Jak/Stat Pathway and Ameliorates Collagen-Induced Arthritis in Rats. In J Pharmacol Exp Ther on 2020 May by Wen-Xiang Cheng, Shan Zhong, et al..PMID: 32029577, , (2020),
PMID: 32029577
et al,Visfatin Induces the Apoptosis of Endothelial Progenitor Cells via the Induction of Pro-Inflammatory Mediators Through the NF-魏B Pathway.Int J Mol Med on 2017 Sep by Lina Sun , Shuchun Chen et al..PMID: 28677720, , (2017),
PMID: 28677720
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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