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位置: 首页 > 细胞因子 > Recombinant Human EPO-a (rHu EPO-a)

Recombinant Human EPO-a (rHu EPO-a)#70204

Recombinant Human EPO-a (rHu EPO-a)
是否有货: 有货
产品总价:
产品详情

产品名称Recombinant Human EPO-a (rHu EPO-a)

简述Recombinant Protein

纯化> 98 % by SDS-PAGE and HPLC analyses.

种属反应性Hu

生物活性Fully biologically active when compared to standard. The Specific Activity was measured by the stimulation of reticulocyte production in normocyth-aemic mice and is no less than 1.5 ⅹ 105 IU mg.

内毒素Less than 0.01 EU/��g of rHuEPO-�� as determined by LAL method.

基因/蛋白名称rHu EPO-a

别名Epoetin

数据库入口号accession:P01588
GeneID:2056

UniprotP01588

计算分子量Mature human EPO, containing 1

实际分子量Sterile Filtered White lyophil

序列APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR

配方Lyophilized from a 0.2 ��m filtered concentrated solution in sodium citrate buffer (1 liter of ddH2O containing 5.9 g of sodium citrate, 5.8 g of sodium chloride and 0.06 g of citric acid).

配置We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg mL. Stock solutions should be apportio

保存This lyophilized preparation is stable at 2-8 ˚C, but should be kept at -20 ˚C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 ˚C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 ˚C to -70 ˚C. Avoid repeated freeze thaw cycles.

背景

Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The production of EPO by kidney cells is increased in response to hypoxia or anemia. Recombinant EPO has been approved for the treatment of anemia associated with chronic renal failure as well as for anemia of AZT treated AIDS patients.

背景文献

1. Hanicki Z. 1988. Pol Arch Med Wewn, 80: 290-3. 2. Haniu M, Narhi LO, Arakawa T, et al. 1993. Protein Sci, 2: 1441-51. 3. Baranowska-Daca EandKsiazek A. 1994. Ann Univ Mariae Curie Sklodowska Med, 48 Suppl 3: 51-60. 4. Chow KM, Szeto CC, Li PK. 2003. Am J Kidney Dis, 41: 266-7; author reply 7. 5. Maurer MH, Schabitz WR, Schneider A. 2008. Curr Med Chem, 15: 1407-11.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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