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位置: 首页 > 磷酸化抗体 > NFκB-p100(Phospho-Ser870) Antibody

NFκB-p100(Phospho-Ser870) Antibody#11016

NFκB-p100(Phospho-Ser870) Antibody
NFκB-p100(Phospho-Ser870) Antibody
NFκB-p100(Phospho-Ser870) Antibody
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产品总价:
产品详情

产品名称NFκB-p100(Phospho-Ser870) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of NFκB-p100 only when phosphorylated at serine 870.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 870(Y-G-S(p)-Q-S) derived from Human NFκB-p100.

基因/蛋白名称NFκB-p100

修饰Phospho

别名p52; p105; H2TF1; LYT10; NF-kB2

数据库入口号Swiss-Prot: Q00653
NCBI Gene ID: 4791
NCBI mRNA: NM_001077494.2
NCBI Protein: NP_001070962.1

UniprotQ00653

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C

应用详情
 Predicted MW: 120kd
Western blotting: 1:500
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Immunofluorescence staining of methanol-fixed HeLa cells using NF-κB p100(phospho-Ser870) antibody (#11016, Red).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p100(phospho- Ser870) antibody (#11016).
Western blot analysis of extract from MDA-MB-435 cells untreated or treated with TNF-α (20ng/ml, 5min) using NF-κB p100(phospho-Ser870) antibody (#11016).
背景

 NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Dobrzanski P., Ryseck R.P., Bravo R.EMBO J. 13:4608-4616(1994)

Betts J.C., Nabel G.J.Mol. Cell. Biol. 16:6363-6371(1996)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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