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Rb(Ab-780) Antibody#21110

Rb(Ab-780) Antibody
Rb(Ab-780) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Rb(Ab-780) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of taotal Rb protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa. 778~782 (T-L-S-P-I) derived from Human Rb.

基因/蛋白名称Rb

别名pRb

数据库入口号Swiss-Prot: P06400
NCBI Protein: NP_000312.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from JK cells using Rb(Ab-780) Antibody #21110.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Ab-780) Antibody #21110(left) or the same antibody preincubated with blocking peptide(right).
背景
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Roesch A, et al.(2005) Mod Pathol. 18(4): 565-572.
Huang H, et al.(2004) Oncogene. 23(12): 2161-2176.
Kitagawa M, et al.(1996)EMBO J. 15(24): 7060-7069.

如果您使用该产品21110发表了文章,请通知我们,让我们可以引用您的文献。

et al,The reversal effect of Ginsenoside Rh2 on drug resistance in human colorectal carcinoma cells and its mechanism. In Hum Cell.On 2018 Jul by Liu GW, Liu YH et al..PMID:29582366, , (2018),
PMID: 29582366
et al,EEN regulates the proliferation and survival of multiple myeloma cells by potentiating IGF-1 secretion.In Biochem Biophys Res Commun on 2014 May 2 by Er-Wen Huang , Sheng-Jiang Xue et al ..PMID: 24704450 , , (2014),
PMID: 24704450
et al,T-helper-1-cell Cytokines Drive Cancer Into Senescence. In Nature on 2013 Feb 21 by Heidi Braum眉ller, Thomas Wieder, et al..PMID: 23376950, , (2013),
PMID: 23376950
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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