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YWHAG Antibody#31246

YWHAG Antibody
YWHAG Antibody
是否有货: 有货
产品总价:
产品详情

产品名称YWHAG Antibody

来源种属Rabbit

克隆性Polyclonal

应用ELISA WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total YWHAG protein.

免疫原类型Peptide

免疫原描述Synthetic peptide corresponding to a region derived from 141-154 amino acids of Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide

基因/蛋白名称YWHAG

别名tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, 14-3-3GAMMA

数据库入口号Genbank No.: NP_036611

UniprotNP_036611

配方Supplied at 3.6mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Predicted MW: 28kd
ELISA: 1:2000-1:10000
Western blotting: 1:1000-1:5000
Immunohistochemistry: 1:50-1:200
Gel: 10%SDS-PAGE
Lane1: Hela cell lysate
Lane2: 293T cell lysate
Lane3: Jurkat cell lysate
Lysates: 40 ug per lane
Primary antibody: 1/1800 dilution
Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Exposure time: 10 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 31246 (YWHAG Antibody) at dilution 1/100, on the right is treated with the synthetic peptide.
背景
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

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400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com