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位置: 首页 > 抗体 > Histone H3.1(Ab-10) Antibody

Histone H3.1(Ab-10) Antibody#21137

Histone H3.1(Ab-10) Antibody
Histone H3.1(Ab-10) Antibody
Histone H3.1(Ab-10) Antibody
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产品总价:
产品详情

产品名称Histone H3.1(Ab-10) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total Histone H3.1 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.8~12 (R-K-S-T-G) derived from Human Histone H3.1.

基因/蛋白名称Histone H3.1

别名H3/a; H3/c; H3/d; H3/f; H3/h

数据库入口号Swiss-Prot: P68431
NCBI Protein: NP_003521.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 17kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from 293 cells using Histone H3.1(Ab-10) Antibody #21137.
Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1(Ab-10) Antibody #21137.
Western blot analysis of extracts of various cell lines, using Histone H3.1(Ab-10) Antibody #21137
背景

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
Cheung, P. et al. (2000) Cell 103, 263-71.

如果您使用该产品21137发表了文章,请通知我们,让我们可以引用您的文献。

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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